Cul./Sens. – Sputum

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The Cul./Sens. – Sputum Test (Sputum Culture and Sensitivity Test) is a laboratory test used to detect and identify bacterial, fungal, or other pathogenic organisms present in sputum (mucus coughed up from the lungs or lower airways). After identifying the organism, sensitivity testing is performed to determine the most effective antibiotics for treatment.

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    Description

    Why It’s Done:

    This test is commonly recommended when a person shows symptoms of a respiratory tract infection, especially when:
    • The infection is chronic, severe, or not responding to initial treatment.
    • There are symptoms such as persistent cough, fever, chest pain, difficulty breathing, or productive cough with discolored mucus.
    • Conditions like pneumonia, tuberculosis, bronchitis, or lung abscesses are suspected.
    Purpose of the test:
    • Identify the cause of a lower respiratory tract infection.
    • Determine which antibiotics or antifungal medications will be most effective.
    • Guide proper treatment to reduce the misuse of antibiotics.

    Preparation:

    • Collect the sample in the morning, ideally before eating or drinking.
    • Rinse your mouth with water beforehand to reduce contamination from oral bacteria.
    • Take a deep breath, cough deeply from the lungs (not the throat), and expel the sputum into a sterile container provided by the lab.
    • Do not use saliva—ensure it is a deep mucus sample from the lungs.
    • If you’re already on antibiotics, inform your healthcare provider, as it may affect test accuracy.