Afb Smear (Zn + Auramine)

2,500.00

The AFB Smear (ZN + Auramine) test is a microscopic examination used to detect acid-fast bacilli (AFB), primarily Mycobacterium tuberculosis, in clinical specimens such as sputum, body fluids, or tissue samples.
This test uses two staining techniques:
• ZN (Ziehl-Neelsen) stain: A traditional method using carbol fuchsin dye to stain AFB, which appear red against a blue background under a light microscope.
• Auramine stain: A fluorescent stain that binds to the waxy cell walls of AFB, which appear bright yellow or green under a fluorescence microscope. This method is more sensitive and allows faster screening.
The dual use of ZN and Auramine stains improves diagnostic accuracy and sensitivity.

Description

Why It’s Done:

The test is performed to:
• Detect tuberculosis (TB) in suspected cases.
• Provide a rapid diagnosis before culture results are available.
• Monitor treatment response in TB patients.
• Screen for AFB infections in other conditions like nontuberculous mycobacteria (NTM).
• Identify infectious cases for public health intervention.
It is especially useful for diagnosing pulmonary TB through sputum smears, but it can also be used for extrapulmonary samples.

Preparation:

• No special preparation is needed for the test.
• For sputum samples:
o The best sample is an early morning deep cough specimen.
o Rinse your mouth with water before collecting to avoid contamination.
• Samples should be collected in a sterile container and sent promptly to the laboratory.
• Inform your healthcare provider if you are on anti-TB medications.