Afb Smear (Zn + Auramine)

2,500.00

The AFB Smear (ZN + Auramine) test is a microscopic examination used to detect acid-fast bacilli (AFB), primarily Mycobacterium tuberculosis, in clinical specimens such as sputum, body fluids, or tissue samples.
This test uses two staining techniques:
• ZN (Ziehl-Neelsen) stain: A traditional method using carbol fuchsin dye to stain AFB, which appear red against a blue background under a light microscope.
• Auramine stain: A fluorescent stain that binds to the waxy cell walls of AFB, which appear bright yellow or green under a fluorescence microscope. This method is more sensitive and allows faster screening.
The dual use of ZN and Auramine stains improves diagnostic accuracy and sensitivity.

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    Description

    Why It’s Done:

    The test is performed to:
    • Detect tuberculosis (TB) in suspected cases.
    • Provide a rapid diagnosis before culture results are available.
    • Monitor treatment response in TB patients.
    • Screen for AFB infections in other conditions like nontuberculous mycobacteria (NTM).
    • Identify infectious cases for public health intervention.
    It is especially useful for diagnosing pulmonary TB through sputum smears, but it can also be used for extrapulmonary samples.

    Preparation:

    • No special preparation is needed for the test.
    • For sputum samples:
    o The best sample is an early morning deep cough specimen.
    o Rinse your mouth with water before collecting to avoid contamination.
    • Samples should be collected in a sterile container and sent promptly to the laboratory.
    • Inform your healthcare provider if you are on anti-TB medications.