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Mycobaterium – Afb Stain+Rapid Culture+Pcr

₹3,500.00

15 minutes
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The Mycobacterium – AFB Stain + Rapid Culture + PCR Test is a comprehensive diagnostic panel used for the detection of Mycobacterium species, including Mycobacterium tuberculosis (the causative agent of tuberculosis) and non-tuberculous mycobacteria (NTM). This panel combines multiple diagnostic methods to detect mycobacteria more effectively and efficiently:

1. AFB Stain (Acid-Fast Bacilli Staining):
o A microscopic test where clinical samples (e.g., sputum, tissue, or fluid) are stained using Ziehl-Neelsen or fluorochrome staining to identify acid-fast mycobacteria.
o Quick and low-cost, it provides initial evidence of mycobacterial presence.
2. Rapid Culture:
o Involves culturing a sample in a liquid medium (e.g., BACTEC or MGIT system) to grow and identify viable mycobacteria. It offers faster results than traditional solid culture, with results in 7–14 days.
o Used to confirm infection and help determine species and drug susceptibility.
3. PCR (Polymerase Chain Reaction):
o A molecular technique that amplifies specific DNA sequences unique to Mycobacterium tuberculosis and other mycobacterial species.
o Offers rapid, specific, and sensitive detection, even in smear-negative cases or when bacterial loads are low.

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    Description

    Why It’s Done:
    This combined test panel is used to:
    • Diagnose active tuberculosis or NTM infections, especially in cases where clinical symptoms suggest infection, but traditional methods (e.g., AFB smear) fail to provide clear results.
    • Provide early and accurate detection of mycobacteria to enable prompt treatment and reduce transmission, particularly in individuals at higher risk (e.g., immunocompromised patients).
    • Differentiate between pulmonary and extrapulmonary TB or between TB and other respiratory diseases.
    • Monitor treatment progress and check for recurrence or relapse, particularly in drug-resistant TB cases.
    • Assist in identifying species and help guide appropriate treatment decisions.

    Preparation:
    • No special fasting or preparation is typically required.
    • A sample collection is needed, depending on the site of infection:
    o Sputum (for lung infections)
    o Tissue biopsies, body fluids, or lymph node aspirates (for extrapulmonary infections)
    • The sample should be collected in a sterile container and sent to the lab promptly to prevent contamination.
    • Inform the healthcare provider if you have recently started TB treatment or have been exposed to individuals with tuberculosis.

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