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Hiv I &Ii Ab Western Blot

₹3,200.00

15 minutes
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The Western Blot test for HIV is a confirmatory test used to detect antibodies against specific HIV-1 and HIV-2 proteins in the blood.
• It follows positive or equivocal results from an initial HIV ELISA or rapid antibody test.
• The Western blot separates HIV viral proteins by electrophoresis, then transfers them to a membrane where they are exposed to the patient’s serum. If antibodies to HIV are present, they will bind to specific proteins (e.g., gp120, gp41, p24), which are then visualized.

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    Description

    Why It’s Done (Purpose):

    1. Confirm HIV Infection:
    o It verifies a positive HIV screening test (like ELISA) to avoid false positives.
    2. Differentiate Between HIV-1 and HIV-2:
    o It can help distinguish whether the antibodies are specific to HIV-1, HIV-2, or both, which can affect treatment decisions.
    3. Legal and Medical Documentation:
    o Used in certain legal, medical, or immigration settings to document HIV status.
    Note: As of recent guidelines (e.g., CDC in the U.S.), Western blot has largely been replaced by newer confirmatory methods like HIV-1/HIV-2 differentiation immunoassays and nucleic acid tests (NATs) because of greater speed and accuracy.

    Preparation Guidelines:

    • No special preparation is needed.
    • Fasting is not required.
    • A blood sample is drawn from a vein.
    • Inform your doctor about:
    o Any recent possible exposure to HIV.
    o Use of antiretroviral medications, PrEP, or PEP, which may affect test sensitivity.

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